Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody (GTX133261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 1 antibody (GTX133261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.