Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 6 antibody [HL1669] (GTX637268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 6 antibody [HL1669] (GTX637268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 Âμg) was separated by 10% SDS-PAGE, and the membrane was blotted with GABA A Receptor alpha 6 antibody [HL1669] (GTX637268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.