Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX132657) diluted at 1:1000.

GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GABARAP protein stained by GABARAP antibody (GTX132657) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.