Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD65 + GAD67 antibody [HL1181] detects GAD65 + GAD67 protein at cytosol and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 + GAD67 stained by GAD65 + GAD67 antibody [HL1181] (GTX636487) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).