GAD65 antibody [HL1187]

Cat. No. GTX636493
Cat. No.  GTX636493
Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493
GTX636493 ICC/IF Image

GAD65 antibody [HL1187] detects GAD65 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
GTX636493 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
GTX636493 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
GTX636493 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
GTX636493 IHC-P Image

GAD65 antibody [HL1187] detects GAD65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
GAD65 stained by GAD65 antibody [HL1187] (GTX636493) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
GTX636493 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
GTX636493 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD65 antibody [HL1187] (GTX636493) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

9 / 9
Anti-GAD65 antibody [HL1187] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in IHC (Paraffin sections) (IHC-P). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
Anti-GAD65 antibody [HL1187] used in Western Blot (WB). GTX636493
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL1187
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P IHC-Fr
  • Reactivity
    Human, Mouse, Rat, Zebrafish, Cat, Dog
See all GAD65 products