Human tissue extract (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody [HL1095] detects GAD67 protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1095] (GTX636279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody [HL1095] detects GAD67 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody [HL1095] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody [HL1095] detects GAD67 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
GAD67 stained by GAD67 antibody [HL1095] (GTX636279) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min