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GAP43 antibody

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101535
Anti-GAP43 antibody used in Western Blot (WB). GTX101535
Anti-GAP43 antibody used in Western Blot (WB). GTX101535
Anti-GAP43 antibody used in Western Blot (WB). GTX101535

Cat. No. GTX101535

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GAP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

IMR32 , mouse hippocampus , rat hippocampus

Predict Reactivity

Bovine, Cat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth associated protein 43 , B-50 , PP46

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse

Background

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GAP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101535

GTX101535 ICC/IF Image

GAP43 antibody detects GAP43 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAP43 protein stained by GAP43 antibody (GTX101535) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-GAP43 antibody used in Western Blot (WB). GTX101535

GTX101535 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX101535 diluted at 1:1000

Anti-GAP43 antibody used in Western Blot (WB). GTX101535

GTX101535 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAP43 antibody used in Western Blot (WB). GTX101535

GTX101535 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

GAP43 antibody Cat. No. GTX101535

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-GAP43 antibody used in Western Blot (WB). GTX101535
Date : Anonymous submitted on 14-Feb-2012
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat brain
Amount used : 37.5μg
Blocking : 5% non-fat milk, 4°C, 16Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169