Immunoprecipitation of GAPDH from rat heart extract using anti-GAPDH MAb 6C5(GTX28245) (A).
Mixture of protein A-Sepharose with anti-GAPDH MAbs and tissue extract was incubated for 30 min at room temperature and precipitated by centrifugation. Pellet was washed with PBS, suspended in reducing electrophoresis sample buffer and heated for 5 minutes at 100 oC. After centrifugation supernatant was loaded on gel and proteins were separated by SDS electrophoresis.
Lane 1: Human GAPDH (1 μg)
Lane 2: GAPDH immunoprecipitated from rat heart tissue extract
Lane 3: Only GTX28245 preincubated with Protein A Sepharose
Lane 4: Only Protein A Sepharose

Heart extracts were prepared from different animal species. About 0.5 mg of homogenized wet cardiac tissue per track was loaded.
Lane 1: isolated human GAPDH, 0.5 μg
Lane 2: human heart tissue extract
Lane 3: pig heart tissue extract
Lane 4: goat heart tissue extract
Lane 5: bovine heart tissue extract
Lane 6: dog heart tissue extract
Lane 7: mouse heart tissue extract
Lane 8: rat heart tissue extract
Lane 9: rabbit heart tissue extract

Immunostaining of GAPDH in A-10 cell line (rat aortic smooth muscle cells). Cells were fixed by formalin and GAPDH was stained by:
GTX28245 (green colour)
F-actin microfilaments-bindingdye (red)
DNA-binding dye (dark blue)

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Sci Rep in 2019. PMID: 31685876

The data was published in the journal Sci Rep in 2019. PMID: 31685876

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2017. PMID: 28139683

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Nat Commun in 2020.PMID: 32647127

The data was published in the journal Front Cell Neurosci in 2019.PMID: 31474830