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GAPDH antibody

Anti-GAPDH antibody used in Western Blot (WB). GTX82899
Anti-GAPDH antibody used in Western Blot (WB). GTX82899

Cat. No. GTX82899

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog, Primate
Package
100 μg ($399)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic peptide: V(126) M G V N H E K Y(140)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_625904

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , BARS-38 , Gapd

Cellular Localization

Cytoplasm, cytosol,Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody used in Western Blot (WB). GTX82899

GTX82899 WB Image

WB analysis of whole cell extracts (30 μg lysate) of A-431 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), PC-3 (Lane 4) and tissue extract (30 μg lysate) of Ms Brain (Lane 5) using GTX82899 GAPDH antibody.
Dilution : 1:1000

Anti-GAPDH antibody used in Western Blot (WB). GTX82899

GTX82899 WB Image

WB analysis of 25 ug of Hela (Lane 1), 293 (Lane 2), HUVEC (Lane 3) and PC12 (Lane 4) cell lysates using GTX82899 GAPDH antibody.
Dilution : 1:3000

REFERENCE

Application Reference

Nakayama I et al. J Biochem 2020; (Epub) Regulation of Epidermal Growth Factor Receptor Expression and Morphology of Lung Epithelial Cells by Interleukin-1β.
Application : WB
Ng MC et al. Fish Physiol Biochem 2012; 38 (4) : 1099-106 Effect of MK-801-induced impairment of inhibitory avoidance learning in zebrafish via inactivation of extracellular signal-regulated kinase (ERK) in telencephalon.
Application : WB
Reactivity : Zebrafish
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399