ICC/IF analysis of SH-SY5Y cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

WB analysis of 15 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX30600 GATA3 antibody [1A12-1D9].
Dilution : 1:1000

IHC-P analysis of human breast carcinoma tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

IHC-P analysis of mouse brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

IP analysis of SH-SY5Y cell lysates using GTX30600 GATA3 antibody [1A12-1D9].
IP reaction : 5μl antibody / 250μg lysate
Dilution : 1:1000

IHC-P analysis of rat brain tissue using GTX30600 GATA3 antibody [1A12-1D9].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

ICC/IF analysis of MCF-7 cells using GTX30600 GATA3 antibody [1A12-1D9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature