Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GCN5L2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunofluorescence of HeLa cells were stained by monoclonal anti-human GCN5L2 antibody (1:500) with Alexa 488 (Green).