Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

GEF-H1 antibody [GT1132] detects GEF-H1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
5 % SDS-PAGE
GEF-H1 antibody [GT1132] (GTX629399) dilution: 1:1000

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min