Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:3000 and competitor's antibody (CST 34001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Indirect ELISA analysis was performed by coating the plate with recombinant full-length human GFAP protein (52.42-0.82 nM). Coated protein was probed with GFAP antibody [HL1307] (GTX636725) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody [HL1307] (GTX636725) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GFAP antibody [HL1307] detects GFAP protein at glia cells by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

GFAP antibody [HL1307] detects GFAP protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GFAP antibody [HL1307] detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GFAP stained by GFAP antibody [HL1307] (GTX636725) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min