Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GIRK1 antibody [N3C3] (GTX108745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded DU145 xenograft, using GIRK1(GTX108745) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min