IHC-P analysis of human colon using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100

IHC-P analysis of mouse liver tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100

IHC-P analysis of rat ovary tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100

IHC-P analysis of rat brain tissue using GTX0424 GLI3 antibody.
Antigen retireval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:100

WB analysis of various tissue lysates using GTX0424 GLI3 antibody.
Loading : 25 μg
Dilution : 1:1000
Blocking buffer: 3% nonfat dry milk in TBST