Immunohistochemical analysis of paraffin-embedded human hepatoma, using GLUD1 + GLUD2 antibody (GTX112361) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse testis
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GLUD1 + GLUD2 antibody detects GLUD1 + GLUD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GLUD1 + GLUD2 protein stained by GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.