GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using GLUD1 + GLUD2 antibody (GTX105765) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GLUD1+ GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Glutamate Dehydrogenase protein stained by GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000.

GLUD1 + GLUD2 antibody detects Glutamate Dehydrogenase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Glutamate Dehydrogenase stained byGLUD1 + GLUD2 antibody (GTX105765) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX105765) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2018. PMID: 29555943