Unboiled U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

GLUT3 antibody detects GLUT3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLUT3 stained by GLUT3 antibody (GTX129175) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:2000.