Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.