Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GNB1 antibody (GTX114442) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GNB1 antibody (GTX114442) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNB1 protein stained by GNB1 antibody (GTX114442) diluted at 1:500.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(GTX114442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 μg of GNB1 antibody (GTX114442).
Western blot analysis was performed using GNB1 antibody (GTX114442).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114442 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNB1 antibody (GTX114442) at 1:300 dilution.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis (whole mount) of zebrafish embryo, using GNB1 antibody (GTX114442) at 1:200 dilution.

The data was published in the journal eNeuro in 2018. PMID: 29911170

The data was published in the journal Front Physiol in 2019. PMID: 31992990