GPR30 antibody [C2C3], C-term detects GPR30 protein by immunofluorescent analysis.
Sample: DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GPR30 antibody [C2C3], C-term detects GPR30 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.(WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GPR30 antibody [C2C3], C-term detects GPR30 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded human endometrial, using GPR30(GTX107748) antibody at 1:150 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GPR30 antibody [C2C3], C-term detects GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GPR30 stained by GPR30 antibody [C2C3], C-term (GTX107748) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPR30 antibody detects GPR30 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPR30 stained by GPR30 antibody (GTX107748) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal PLoS One in 2013.PMID: 24039841

The data was published in the journal PLoS One in 2013.PMID: 24039841

The data was published in the journal PLoS One in 2013.PMID: 24039841

The data was published in the journal PLoS One in 2013.PMID: 24039841