Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GPX2 antibody [C1C3] detects GPX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody [C1C3] (GTX100292) diluted by 1:1000

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPX2 antibody [C1C3] detects GPX2 protein at granule by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GPX2 antibody detects GPX2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody (GTX100292) diluted by 1:500

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] (GTX100292).
Western blot analysis was performed using GPX2 antibody [C1C3] (GTX100292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min