Wild-type (WT) and GRB2 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRB2 antibody (GTX100294) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GRB2 antibody detects GRB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GRB2 antibody detects GRB2 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GRB2 antibody detects GRB2 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GRB2 antibody detects GRB2 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRB2 antibody (GTX100294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GRB2 antibody detects GRB2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GRB2 antibody detects GRB2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRB2 protein stained by GRB2 antibody (GTX100294) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
12% SDS PAGE
GTX100294 diluted at 1:1000

GRB2 antibody detects GRB2 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GRB2 antibody (GTX100294) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min