GRK2 antibody [N3C3-2] detects GRK2 protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
GRK2 antibody [N3C3-2] (GTX107915) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [N3C3-2] (GTX107915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107915 diluted at 1:1000