WB analysis of UV-treated HEK293T (A), mouse liver (B), rat liver (C), H2O2-treated PC12 (D) whole cell lysates using GTX32191 GSK3 alpha (phospho Ser21) antibody.

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32191 GSK3 alpha (phospho Ser21) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

ICC/IF analysis of formalin-fixed PC12 cells using GTX32191 GSK3 alpha (phospho Ser21) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes