Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX111192 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the 2020 in Front Cell Dev Biol. PMID: 33015048