Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta (phospho Ser9) antibody [GT1209] (GTX00971) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of NIH-3T3 cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209].
Dilution : 1:1000
Loading : 25μg

IHC-P analysis of human colon carcinoma tissue section using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209].
Dilution : 1:100

WB analysis of HeLa cell extracts with/without Calyculin A (100 nM, 37ºC, 30 min) treatment after serum-starvation overnight using GTX00971 GSK3 beta (phospho Ser9) antibody [GT1209].
Dilution : 1:1000
Loading : 25μg