Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with GSX2 antibody [HL2533] (GTX638902) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GSX2 antibody [HL2533] detects GSX2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 embryo.
GSX2 stained by GSX2 antibody [HL2533] (GTX638902) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min