Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Galactosidase alpha(GTX101178) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Galactosidase alpha antibody [N1C2] detects Galactosidase alpha protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of GLA protein from 293T whole cell extracts using 5 μg of Galactosidase alpha antibody [N1C2] (GTX101178).
Western blot analysis was performed using Galactosidase alpha antibody [N1C2] (GTX101178) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.