Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635151 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Galectin 3 antibody [GT1473] detects Galectin 3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Galectin 3 stained by Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.