Wild-type (WT) and LGALS3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Galectin 3 antibody [HL2020] detects Galectin 3 protein by immunofluorescent analysis.
Sample: MDCK cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat stomach.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Galectin 3 antibody [HL2020] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Galectin 3 stained by Galectin 3 antibody [HL2020] (GTX637921) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min