Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin 8 antibody (GTX117687) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Galectin 8(GTX117687) antibody at 1:200 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Galectin 8 antibody detects Galectin 8 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human LGALS8-transfected 293T cells
10% SDS-PAGE
Galectin 8 antibody (GTX117687) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.