Germ cell-specific antigen antibody [TRA98]

Cat. No. GTX00717
References 3
Cat. No.  GTX00717
Orthogonal
Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Paraffin sections) (IHC-P). GTX00717
GTX00717 IHC-P Image

IHC-P analysis of mouse testis tissue section (10-day-old) using GTX00717 Germ cell-specific antigen antibody [TRA98]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:400
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

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Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Frozen sections) (IHC-Fr). GTX00717
GTX00717 IHC-Fr Image

IHC-Fr analysis of mouse testis tissue section (7-day-old) using GTX00717 Germ cell-specific antigen antibody [TRA98].
Scale bar : 50 μm

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Anti-Germ cell-specific antigen antibody [TRA98] used in Western Blot (WB). GTX00717
GTX00717 WB Image
Orthogonal

This protien is testis-specific.

WB analysis of various mouse tissue samples using GTX00717 Germ cell-specific antigen antibody [TRA98].
This protien is testis-specific.
Dilution : 1 μg/ml
Loading : 100 μg

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Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Paraffin sections) (IHC-P). GTX00717
Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Frozen sections) (IHC-Fr). GTX00717
Anti-Germ cell-specific antigen antibody [TRA98] used in Western Blot (WB). GTX00717
  • Host
    Rat
  • Clonality
    Monoclonal
  • Clone Name
    TRA98
  • Isotype
    IgG2a
  • Applications
    WB ICC/IF IHC-P IHC-Fr FCM IP IHC
  • Reactivity
    Mouse
Summary
This antibody recognizes a mouse testicular germ cell-specific antigen. In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with this antibody. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells.
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