Gli2 antibody

Cat. No. GTX27195
Cat. No.  GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195
GTX27195 WB Image

WB analysis of mouse brain tissue lysate using GTX27195 Gli2 antibody prior incubated with (lane 2) or without (lane 1) blocking peptide.
Loading : 25 μg
Dilution : 1:750

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Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
GTX27195 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

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Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
GTX27195 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

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Anti-Gli2 antibody used in Western Blot (WB). GTX27195
GTX27195 WB Image

Western blot using GeneTex's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

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Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.

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Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

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Anti-Gli2 antibody used in Western Blot (WB). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB IHC-P IHC-Fr ELISA IHC
  • Reactivity
    Human, Mouse
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