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Glutamine synthetase antibody [GT1055]

KO/KD Overexpression
Anti-Glutamine synthetase antibody [GT1055] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Frozen sections) (IHC-Fr). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Frozen sections) (IHC-Fr). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654

Cat. No. GTX630654

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1055

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Glutamine synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , HeLa , HepG2 , mouse eye , mouse brain , rat brain , DDDDK-tagged GLUL-transfected 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888230

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Glutamine synthetase antibody [GT1055] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630654

GTX630654 ICC/IF Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Glutamine synthetase antibody [GT1055] used in IHC (Frozen sections) (IHC-Fr). GTX630654

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500.

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Frozen sections) (IHC-Fr). GTX630654

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500. Blue: DAPI

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630654) diluted at 1:1000.

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutamine synthetase antibody [GT1055] used in Western Blot (WB). GTX630654

GTX630654 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

Anti-Glutamine synthetase antibody [GT1055] used in IHC (Paraffin sections) (IHC-P). GTX630654

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Jin-Wei Jhu et al. Int J Mol Sci 2021; 22 (18) SREBP1-Induced Glutamine Synthetase Triggers a Feedforward Loop to Upregulate SREBP1 through Sp1 O-GlcNAcylation and Augments Lipid Droplet Formation in Cancer Cells.
Application : WB
Reactivity : Human
Lin D et al. Cells 2021; 10 (4)
Application : WB
Reactivity : Human
Seki T et al. Mol Cell Neurosci 2018; 93 : 36-47 d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations