Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:400.
Blue: Hoechst 33342 staining.

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.