WB analysis of mouse cardiac myocyte lysate using GTX22479 Glycogen synthase 1 (phospho Ser641) antibody.
Lane 1 : Mock
Lane 2 : 10 nM insulin-treated whole cell lysate
Lane 3 : 100 nM insulin-treated whole cell lysate
1 nM insulin-treated whole cell lysate
Lane 5 : Mock
Lane 6 : 4 nM CLA-treated whole cell lysate
Lame 7 : 20 nM CLA-treated whole cell lysate
Lane 8 : 100 nM CLA-treated whole cell lysate

IHC-P analysis of human prostate tissue using GTX22479 Glycogen synthase 1 (phospho Ser641) antibody.
Dilution : 5 μg/mL

Immunohistochemistry with Anti-Glycogen Synthase at pS641antibody (GTX22479) Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody: 5 μg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 μM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system.