Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (GTX132672) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right).
Glypican 1 antibody (GTX132672) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min