HepG2 conditioned medium (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Granulins antibody (GTX100803) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

HepG2 conditioned medium (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody (GTX100803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Granulins antibody detects GRN protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Granulins antibody (GTX100803) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min