Hspa5 antibody detects Hspa5 protein by Western blot analysis.
A. 50 μg Rat heart lysate/extract
B. 50 μg Rat Lung lysate/extract
C. 50 μg Rat Liver lysate/extract
D. 50 μg Rat kidney lysate/extract
7.5 % SDS-PAGE
Hspa5 antibody (GTX127934) dilution: 1:10000

Hspa5 antibody detects Hspa5 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody (GTX127934) diluted at a dilution of 1:10000.

Hspa5 antibody detects Hspa5 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Hspa5 antibody (GTX127934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: NIH-3T-3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
7.5% SDS PAGE
GTX127934 diluted at 1:10000

Hspa5 antibody detects Hspa5 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Hspa5 antibody (GTX127934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Hspa5 antibody detects Hspa5 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Hspa5 antibody (GTX127934) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Hspa5 antibody detects Hspa5 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hspa5 protein stained by Hspa5 antibody (GTX127934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

The data was published in the 2018 in Sci Rep. PMID: 29891991

The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510

The data was published in the 2019 in J Clin Biochem Nutr. PMID: 30705510