Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HADHA antibody [N2C1], Internal detects HADHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
7.5% SDS PAGE
GTX113727 diluted at 1:1000

HADHA antibody [N2C1], Internal detects HADHA protein by Western blot analysis.
A.30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
HADHA antibody [N2C1], Internal (GTX113727) dilution: 1:500

HADHA antibody [N2C1], Internal detects HADHA protein at Mitochondia by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20ºC 100% MeOH for 5 min.
Green: HADHA protein stained by HADHA antibody [N2C1], Internal (GTX113727) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX113727 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HADHA(GTX113727) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal BMC Biochem in 2014. PMID: 25152047