Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HAGH antibody (GTX117595) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HAGH(GTX117595) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min