Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HAI-2 antibody [XY9] (GTX02801) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Unboiled and boiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HAI-2 antibody [XY9] (GTX02801) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.