HAT1 antibody immunoprecipitates HAT1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HAT1 protein by 2 μg of HAT1 antibody (GTX110643) 12% SDS-PAGE The immunoprecipitated HAT1 protein was detected by HAT1 antibody (GTX110643) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded human colon, using HAT1(GTX110643) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

HAT1 antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HAT1 protein stained by HAT1 antibody [N1C3-2] (GTX110643) diluted at 1:500.
Blue: Hoechst 33342 staining.

HAT1 antibody detects HAT1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAT1 antibody (GTX110643) diluted by 1:10000.