GeneTex

  • Country / Location Selection

United States (US)

HAUS6 antibody

Anti-HAUS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118732
Anti-HAUS6 antibody used in Western Blot (WB). GTX118732
Anti-HAUS6 antibody used in Western Blot (WB). GTX118732
Anti-HAUS6 antibody used in Western Blot (WB). GTX118732
Anti-HAUS6 antibody used in IHC (Paraffin sections) (IHC-P). GTX118732
Anti-HAUS6 antibody used in Western Blot (WB). GTX118732
Anti-HAUS6 antibody used in Western Blot (WB). GTX118732
Anti-HAUS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118732

Cat. No. GTX118732

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HAUS6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

Jurkat , 293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HAUS6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618597

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HAUS augmin like complex subunit 6 , Dgt6 , FAM29A

Cellular Localization

Cytoplasm , cytoskeleton , Spindle , Centrosome

Background

HAUS6 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HAUS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118732

GTX118732 ICC/IF Image

HAUS6 ( IFA using GTX118732 at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.

Anti-HAUS6 antibody used in Western Blot (WB). GTX118732

GTX118732 WB Image

Sample (30 μg of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX118732 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HAUS6 antibody used in Western Blot (WB). GTX118732

GTX118732 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HAUS6 antibody used in Western Blot (WB). GTX118732

GTX118732 WB Image

HAUS6 antibody detects HAUS6 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HAUS6 antibody (GTX118732) diluted by 1:1000.

Anti-HAUS6 antibody used in IHC (Paraffin sections) (IHC-P). GTX118732

GTX118732 IHC-P Image

HAUS6 antibody detects HAUS6 protein on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
HAUS6 antibody (GTX118732) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HAUS6 antibody used in Western Blot (WB). GTX118732

GTX118732 WB Image

The data was published in the journal J Cell Sci in 2018.PMID: 29514856

Anti-HAUS6 antibody used in Western Blot (WB). GTX118732

GTX118732 WB Image

The data was published in the journal J Cell Sci in 2018.PMID: 29514856

Anti-HAUS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118732

GTX118732 ICC/IF Image

The data was published in the journal J Cell Sci in 2018.PMID: 29514856

REFERENCE

Application Reference

Ustinova K et al. J Cell Sci 2023; 136 (12) Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP.
Larsson VJ et al. J Cell Sci 2018; (Epub) Mitotic spindle assembly and γ-tubulin localisation depend on the integral nuclear membrane protein, Samp1.
Application : WB, ICC/IF
Reactivity : Human
Tsai CY et al. J Biol Chem 2011; 286 (34) : 30097-106 Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.
Submit a Reference

REVIEW

There are currently no reviews for HAUS6 antibody (GTX118732). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169