Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HAX1 antibody [HL2170] (GTX638174) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HAX1 antibody [HL2170] detects HAX1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HAX1 stained by HAX1 antibody [HL2170] (GTX638174) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min