Sample (30 μg of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX114922 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

A549 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term (GTX114922) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with HCF1 antibody [N1], N-term (GTX114922) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.