IHC-Fr analysis of mouse  hypothalamus tissue using GTX54824 HCN2 antibody. (V = Third ventricle)
Panel A : HCN2 (red) appears in cells of the paraventricular nucleus (PVN, arrows).
Panel B : Staining of paraventricular nerve cells with mouse anti-calcium binding protein (CBD28k, green).
Panel C : Merge of HCN2 and CBD28k demonstrates some co-localization.

IHC-Fr analysis of mouse thalamus tissue using GTX54824 HCN2 antibody.
Panel A : Staining of HCN4 (green) appears in the ventral posterior thalamic nucleus (VPL).
Panel B : In the same section as in A, staining of HCN2 (red) appears in the ventral posterior thalamic nucleus (VPL) and also in the reticular thalamic nucleus (RT). The area between these thalamic nuclei (star) is white matter and neither protein is expressed in that region.
Panel C : Merged images of A and B.

WB analysis of rat brain membrane lysate using GTX54824 HCN2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

ICC/IF analysis of PFA-fixed rat DRG primary cells using GTX54824 HCN2 antibody.
Green : Primary antibody
Blue : DNA dye Hoechst 33342
Dilution : 1:100

IHC-Fr analysis of rat cerebellum tissue using GTX54824 HCN2 antibody.
Panel A : HCN2 (red) appears in Purkinje cells (arrows).
Panel B : Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green) demonstrates the restriction of HCN2 to neuronal cell bodies.
Panel C : Merge of HCN2 and GFAP images demonstrates the respective localization of these proteins.