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HDAC4 (phospho Ser632) antibody

Anti-HDAC4 (phospho Ser632) antibody used in Western Blot (WB). GTX50237

Cat. No. GTX50237

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Human, Rat
Package
100 μl ($399)
See all HDAC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 632 (A-Q-S(p)-S-P) derived from human HDAC4.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11168263

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 4 , AHO3 , BDMR , HA6116 , HD4 , HDAC-4 , HDAC-A , HDACA

Cellular Localization

Nucleus,Cytoplasm

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HDAC4 (phospho Ser632) antibody used in Western Blot (WB). GTX50237

GTX50237 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with EGF (lane 2) using GTX50237 HDAC4 (phospho Ser632) antibody.

REFERENCE

Application Reference

Zhao B et al. Front Pharmacol 2022; 13 : 1010483 Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation.
Lai CY et al. Neuropharmacology 2018; 135 : 536-546 GluN2B/CaMKII mediates CFA-induced hyperalgesia via HDAC4-modified spinal COX2 transcription.
Application : WB
Reactivity : Rat
Lin TB et al. J Pineal Res 2016; Melatonin relieves neuropathic allodynia through spinal MT2-enhanced PP2Ac and downstream HDAC4 shuttling-dependent epigenetic modification of hmgb1 transcription.
Application : WB
Reactivity : Rat
Lin TB et al. Anesthesiology 2015; 123 (1) : 199-212 Modulation of Nerve Injury-induced HDAC4 Cytoplasmic Retention Contributes to Neuropathic Pain in Rats.
Application : WB, IHC-Fr, IP
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399