WB analysis of LPS-treated HEK293T (A), TNFa-treated Raw264.7 (B), rat kidney (C) whole cell lysates using GTX32287 HDAC5 (phospho Ser498) antibody.

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32287 HDAC5 (phospho Ser498) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32287 HDAC5 (phospho Ser498) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)